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1B8-F11
1B8-F11
規(guī)格:
貨期:
編號(hào):B163617
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 1B8-F11
商品貨號(hào) B163617
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody is specific for the gD glycoprotein of BHV-1 and neutralizes the virus in the absence of complement.
It is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine herpesvirus I (BHV-1); also known as infectious bovine rhinotracheitis virus (IBRV)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine herpesvirus I (BHV-1); also known as infectious bovine rhinotracheitis virus (IBRV)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with BHV-1.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the gD glycoprotein of BHV-1 and neutralizes the virus in the absence of complement.
It is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal: Two to three times weekly.
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor CA Whetstone
Deposited As mouse (B cell); mouse (myeloma)
References

. . Conf. Res. Workers Anim. Dis. : 54, 1987.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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